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"ATA-test" -  laboratory system for examination of albumin functional activity


Laboratory test of a functional activity of serum albumin: for early detection of septic complications and intoxications; for detection and monitoring of malignant activity.

The scope of the test

  • early diagnostics (1-2 days after surgery) and monitoring of septic complications
  • diagnostics of growth of malignant tumors of different types and localizations
  • monitoring of anti-cancer treatment  in order to adjust therapy, relapse control.

The main advantages

  • small volume of  0.2 ml serum required
  • fresh samples can be analyzed within 5 days after collection
  • frozen serum and EDTA plasma samples can be stored for a long time and analyzed within 2 hours after those were thawed
  • simple preparation of investigated samples
  • short time of 20 minutes to get the test result
  • capacity of Analyzer is 15 samples per hour
  • high sensitivity and specificity of the test

Equipment and consumables for the "ATA-test":

  • laboratory analyzer "EPR AXM-09" (CE Certificate of Conformity No. LS.08.02.2153)
  • reagent kit "ATA-test"
  • Standard laboratory equipment:
    • Centrifuge providing RFC 1000-1500 g
    • microtiterplate shaker
    • 96-well microtiterplates
    • 10-50 µl pipettor with tips
    • microcapillar pipets on a volume of 40 µl
    • pipette for filling the capillary

Analyzer "EPR AXM-09" and reagent kits "ATA-test", as well as other consumables and supplies for the "ATA-test", are supplied by Scientific and Production Enterprise "Albutran" .


Principle of the method

Assessment of serum albumin functionality parameters is based on spin probe method.

For the test 50 µl of serum or plasma EDTA are mixed with reagents from "ATA-test" containing specific to albumin spin probe. The microtiterplate with the prepared samples are incubated for 10 min in a shaker in order to get equilibrium of spin probe binding to albumin. After incubation the  prepared samples are draw into capillaries and measured at the Analyzer "EPR AXM-09".

The analyzer automatically measures the EPR spectrum of the spin probe, performs spectrum analysis and calculates indexes of functional activity of albumin - binding efficiency (BE), transport efficiency (RTQ), detoxifying efficiency (DTE), and index of disturbance of albumin conformation (DR).

Interpretation of results

For diagnostics of intoxication and septic complications  
(reagent kits "ATA-test T-20" or "ATA-test T-80"):

    • DTE index above 40% indicates a normal condition of albumin transport function
    • DTE below 40% indicates intoxication that could be of various origins
    • DTE in the range of 20-30% indicates moderate level of intoxication
    • DTE below 20% is typical for severe intoxication and/or development of septic complications
    • DTE below 9% is observed in patients developing sepsis or SIRS
    • DTE below 3% is observed in patienss with septic shock

Diagnostics and monitoring of cancer
(reagent kits "ATA-test- -20 "or" ATA-test- -80 :

    • DR index greater than 1.0 indicates that the patient has not an active malignancy
    • DR value in the range from 0 to 1.0 should be seen as borderline
    • DR value below 0 indicates that there is an active malignant growth in the patient

Evaluation of a quality of albumin-containing preparations for infusions,  such as albumin solutions, plasma preparations, etc.
(reagent kits "ATA-test T-20" or "ATA-test T-80"):

    • BE index in the range of 100-135% or higher indicates a high efficacy of metabolites binding by albumin (increased binding constant of the protein), which corresponds to the preparations efficient for detoxification
    • BE index below 65% indicates a decrease of the albumin binding efficacy, which may be the result of a deterioration of the protein conformation during the manufacture or storage of the preparations, due to an excessive concentration of stabilizing reagents, etc.
    • RTQ index in the normal range (60 to 100%) shows a normal balance of albumin transport parameters, the same as it is for native serum albumin of healthy adults. Such preparations should be used for infusion intended to support or supplement the albumin transportation system in the patient
    • RTQ value below 60% indicates that the albumin’s functions of binding and releasing of metabolites are weak.